HHV-6 FOUNDATION REPOSITORY OF REAGENTS
Inventory 11/1/2011
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HHV-6 A Viruses / Infected Cells
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Name |
Original Source |
Primary cell line for Propagation |
Other cell lines for propagation |
| HSB2 / GS | Chandran, B / ABL-Ablashi | HSB2 | J-JHAN; SUPT-1 |
| HSB2 / CO1 | Krueger, G | HSB2 | |
| HSB2 / CO2 | Krueger, G | HSB2 | |
| HSB2 / CO3 | Krueger, G | HSB2 | |
| HSB2 / CO4 | Krueger, G | HSB2 | |
| HSB2 / CO7 | Krueger, G | HSB2 | |
| HSB2 / U1102 | ABL | HSB2 | |
| HHV-6 / WA | Carrigan, D / Knox, K | HSB2 | Cord blood; PBMCs |
| SIE | Agut, H / Bonnafous, P | PHA-stimulated cord blood | |
| HSB-I | Luka, J / Bioworld Antibodies | Latently infected HHV-6A cell line | |
| HSB-M | Luka, J / Bioworld Antibodies | Latently infected HHV-6A cell line | |
| HSB-ML | Luka, J / Bioworld Antibodies | Latently infected HHV-6A cell line | |
| JLO | Luka, J / Bioworld Antibodies | HHV-6 A from CIHHV-6 patient (reactivated) | Molt-3 |
| JL-LCL | Luka, J / Bioworld Antibodies | In-vitro established cell line from CIHHV-6 patient; contains both integrated HHV-6 & EBV | |
| PL-LCL | Luka, J / Bioworld Antibodies | In-vitro established cell line from CIHHV-6 patient; contains both integrated HHV-6 & EBV | |
| DA Isolate | Ablashi, D | HSB2 | SUPT-1 or Molt-3 |
| JJhan/U1102 | Medveczky, P | JJhan | |
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HHV-6 B Viruses / Infected Cells
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Name |
Original Source |
Primary cell line for Propagation |
Other cell lines for propagation |
| SUPT-1 / Z29 | ABL / Ablashi, D. | SUPT-1 | |
| MOLT-3 / Z29 | Pellett, P. | MOLT-3 | Cord blood; PBMCs |
| HST | Mori, Y | MOLT-3 | Cord blood; PBMCs |
| ENO | Mori, Y | MOLT-3 | Cord blood; PBMCs |
| KYO | Mori, Y | MOLT-3 | Cord blood; PBMCs |
| GCVR1 (derived from wildtype HST) | Agut, H / Bonnafous, P | MT4- Resistant to antiviral GCV | |
| CDVR1 (derived from wildtype HST) | Agut, H / Bonnafous, P | MT4- Resistant to antiviral CDV | |
| GPFAR1 (derived from wildtype HST) | Agut, H / Bonnafous, P | MT4- Resistant to antiviral PFA and GVC | |
| 400 | Yoshikawa, T | PHA-stimulated cord blood | |
| 393 | Yoshikawa, T | PHA-stimulated cord blood | |
| 315 | Yoshikawa, T | PHA-stimulated cord blood | |
| 365 | Yoshikawa, T | PHA-stimulated cord blood | |
| 466 | Yoshikawa, T | PHA-stimulated cord blood | |
| MAR | Agut, H / Bonnafous, P | PHA-stimulated cord blood | |
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HHV-7 Viruses / Infected Cells
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Name |
Original Source |
Primary cell line for Propagation |
Other cell lines for propagation |
| SUPT-1 / JI | Ablashi, D | SUPT-1 | Cord blood |
| Sato | Yamanishi, K | PBMCs; SUPT-1 | Cord blood |
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HHV-6 LATE ANTIGEN ANTIBODIES |
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Protein Name |
Clone Name |
Dilutions & Protein Concentration |
Description |
| gp 116/64/54(A&B-specific) | 6A5G3 | IFA titer: >1:1000 IHC: 1:250 to 1:300 Dilution: 1:500Protein Conc: 3.5 mg/ml | This core protein has been most widely used in IHC on oral carcinoma, epilepsy, and MS infected tissues. It reacts with both A and B variant infected cells. |
| gp110(A-specific) | 2E2 | IFA titer: 1:2560 IHC: 1:200 to 1:300 Dilution: 1:500Protein Conc: 3.5 mg/ml | We have used this A-specific late antigen antibody on retina cells collected from HIV-1 positive patients. It detects cells replicating HHV-6A. |
| p98(B-specific) | OHV-3 | IFA titer: 1:4000 ELISA: 1:5000 IHC: 1:500Dilution: 1:200
Protein Conc: 2.0 mg/ml |
Dr. Lautenschlager in Finland finds this antibody to be more useful than gp 116/64/54 in identifying reactivation of HHV-6B in PBMC’s of transplant patients and developed quantitative antigenemia, if it is a gH p98 protein. |
| OHV-1 (gB)(A&B-specific) | OHV-1 | IFA titer: 1:2560 IHC: not titered Dilution: 1:500Protein Conc: 3.5 mg/ml | This mab is associated with viral replication and reacts with both A and B infected cells. |
| gp 82/105(A-specific) | 2-D6 | IFA titer: 1:2560 IHC: 1:200 to 1:400 Dilution: 1:500Protein Conc: 4.0 mg/ml | This is a gQ protein antibody that we have used in tissues where we found viral replication of A. This is a neutralizing mab for HHV-6A. |
| gp102 (late) | 7A2 | IFA titer: 1:1600 | It reacts with A & B and in the cytoplasm of infected cells. |
| p180(A&B-specific) | 4A6 | IFA titer: 1:20 | It reacts with A & B. |
| OHV-2(A&B-specific) | OHV-2 | IFA titer: 1:2560 | It reacts with A & B. |
| HHV-6 LATENT ANTIBODIES | |||
| Protein Name | Clone Name | Dilutions & Protein Concentration | Description |
| U94 | IFA titer: 1:200 WB: 1:1000 Protein Conc: 0.2 mg/ml | This is a latent gene of HHV-6 and reacts with HHV-6B | |
| p37 | IFA titer: >1:1000 IHC: 1:300 Protein Conc: 100 ug/uL | ||
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HHV-6 EARLY ANTIGEN ANTIBODIES |
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Protein Name |
Clone Name |
Dilutions & Protein Concentration |
Description |
| p41(A&B-specific) | 9A5D12 | Protein Conc: 2.5 mg/ml | DNA polymerase processing protein, which appears before viral DNA synthesis. This antibody reacts in the nucleus of the cell. Infected with HHV-6A and HHV-6B variants. It appears 12 to 18 hours after infection. |
| p41/38(A-specific) | C-5 | IFA titer: 1:2560 IHC: 1:300 Dilution: 1:500Protein Conc: 4.0 mg/ml | DNA polymerase processing protein, which appears before viral DNA synthesis. A-specific early antigen. Reacts in the nucleus of the cell (similar to p41) but can also leak out into cytoplasm. |
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HHV-6 IMMEDIATE EARLY ANTIBODIES |
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Protein Name |
Clone Name |
Dilutions & Protein Concentration |
Description |
| Intermediate earlyp6 H8 | IE-2 | IFA titer: 1:200 to 1:400 IHC: 1:10 to 1:20 Protein Conc: 1mg/mL | Monoclonal IE-2, which is specific for HHV-6A. It is also purified IgG from cell culture fluid. This reacts with HHV-6A infected cells. |
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HHV-7 LATE ANTIBODIES |
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Protein Name |
Clone Name |
Dilutions & Protein Concentration |
Description |
| U27 | 11H8 | IFA titer: 1:1000 | It is specific for HHV-7 and It does not cross react with HHV-6. |
| KR4 | KR4 | IFA titer: 1:2560 | It is specific for HHV-7 and It does not cross react with HHV-6. |
| *Please note that titers may vary, however we are giving the IFA titer of 1:1000 where intensity of IFA is + and at 1:500 it is 2 and 3+. Also note that for IHC these are ESTIMATED dilutions. Actual dilutions may vary depending on procedure and the type of tissue used.* | |||
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HHV-6 COSMIDS |
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The following cloned fragments of HHV-6 are available either as bacterial stabs for growing your own DNA or as purified DNA (~10µg/vial) |
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| Name | Vector | Insert (Map on HHV-6 genome) | Insert size (kB) | Remarks |
| pMF147-19 | pWE15-cosmid | 90-130 | ~40 | See Reference 1 |
| pMF147-31 | pWE15-cosmid | 15-52 | ~27 | See Reference 1 |
| pMF210-8 | pWE15-cosmid | 72-110 | ~38 | See Reference 1 |
| pMF228-14 | Bluescript KS-(BamHI) | 2-30 | ~28 | See Reference 1See Note 1 |
| pMF402-27 | pCIN cosmid (SmaI) | 112-155 | ~43 | See note 2 |
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References:
Notes:
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HHV-6 & 7 PLASMIDS |
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Click HERE to see a full list of HHV-6 & 7 Plasmids available. A clone map of HHV-6B strain Z-29 has been provided below for your convenience. Please contact Repository Manager Jill Chase if you are interested in obtaining these or any of the Reagents listed above. |
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