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Grant: Variant Specific Two Step PCR ELISA PI: Prof. Yi-Wei Tang, MD, PhD, Vanderbilt University, USA Prof. Yi-Wei Tang, MD, PhD, Director of the Molecular Infectious Disease Laboratory at Vanderbilt University Medical Center, and his team, are developing a diagnostic and simultaneous differentiation of HHV-6 variants procedure by combining a magnetic bead-based nucleic acid extraction method, a PCR amplification and a colorimetric microtiter plate identification (MAG-PCR-EIA) to enhance the test sensitivity. When the viral load is very low, it is helpful to utilize methods to process larger volumes of clinical specimens and enhance the nucleic acid (DNA and RNA) recovery rate. The magnetic bead nucleic acid extraction method can enhance the extracted nucleic acid quantity and quality as well as handle large specimen volumes, thus improving test sensitivity. Dr. Tang has developed and validated a PCR-EIA system for specifically detecting and identifying HHV-6. The PCR-EIA further enhances test sensitivity with additional signal amplification. Dr. Tang has developed and validated over 40 novel molecular microbiologic techniques for diagnosis and monitoring of infectious for the past 8 years at Vanderbilt including a very sensitive assay for c. pneumonia. Using this assay, Dr. Tang was able to detect one DNA copy of HHV-6 in coded serum spiked samples provided by the Foundation. He was also able to type the specific HHV-6 variant in these samples.
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