Associated Conditions HHV-6 & Multiple Sclerosis

HHV-6 & Multiple Sclerosis

There is growing evidence that HHV-6A plays a direct role in MS, and/or an indirect role as an activator of herpesviruses such as EBV or endogenous retroviruses such as HERV-W.  Here are just a few highlighted findings from the literature on the relationship between HHV-6 infection and MS:

HHV-6A DNA has been found in cell-free specimens from MS patients—but not from healthy controls—and is apparent during relapses more than during remission (Alvarez-Lafuente 2004Chapenko 2003Berti 2002Soldan 1997Alvarez Lafuente 2006).HHV-6 serum DNA levels diminish with interferon beta treatment and patients who do not clear their HHV-6 infection during interferon therapy have a poor prognosis (Garcia Montojo 2011).HHV-6 DNA is found in the spinal fluid of MS patients more frequently than in those with other neurological diseases (Alvarez Lafuente 2008).More HHV-6 DNA is found in MS patient serum during exacerbations than during relapses (Alvarez-Lafuente 2004Behzad-Behbahani 2011).

More HHV-6 DNA is found in MS plaques than in non-affected white matter by immunohistochemistry and in situ in local tissue PCR (Goodman 2003).

Lymphoproliferative response to HHV-6A is greater in MS patients than in controls; 67% of MS patients have significant lymphocyte response to HHV-6 vs 32% of controls (Soldan 2000).

HHV-6 intrathecal antibodies (antibodies generated in the spinal fluid) have been observed in 23% of MS patients but 0% of healthy controls (Derfuss 2005).

23% of 80 patients with oligoclonal bands were found to have HHV-6 specific oligoclonal bands; HHV-6 oligoclonal bands were found at similar rates in other demyelinating neurological diseases (Virtanen 2011).

IgM and IgG antibodies to HHV-6 early antigen (antibodies that are only found during active infection) are significantly greater in MS patients than in controls (Patnaik 1995Soldan 1997Ablashi 2000Khaki 2011).

Investigators at UCSF induced MS-like lesions in marmoset monkeys by injecting them with HHV-6A (International Conference on HHV-6 & 7, 2006). Investigators from the NINDS induced brain lesions and MS-type ambulatory deficits in marmosets after exposing them to intranasal injections of HHV-6A virus (International Conference on HHV-6 & 7, 2011).

New reports suggest that a genetic link between HHV-6 infection and MS could place specific patients with HHV-6 infection at risk for the acquisition of MS (Martinez 2007Vandenbroeck 2011).

Reasons to suspect viruses as a cause of MS:

  • All demyelinating disorders with known etiology have been caused by viruses;
  • Antiviral treatments such as beta interferon have been effective in MS;
  • MS symptoms wax and wane as do viral infections, particularly Herpesvirus family members;
  • MS symptoms worsen with viral infections such as colds;
  • Herpes infections, like MS, flare in response to stress, heat, and other infections;
  • Environmental factors are more important than inherited susceptibility;
  • Geographic outbreaks have been reported.

 

Over the years, a number of individual agents have been investigated for playing a role in MS.  Candidates for triggers of MS have included mycobacterium tuberculosis, measles, mumps, rubella, retroviruses herpes simplex and varicella zoster, adenovirus, coronavirus, and vaccinia virus as well as bacteria such asBorrelia burgdorferi.

Today, attention focuses primarily on HHV-6, Epstein Barr virus (EBV), Chlamydia pneumonia (Cpn) and human endogenous retroviruses (HERVs). Since these viruses and pathogens potentiate each other, it is possible that there are many infections involved in a chain reaction, with the end result being an autoimmune process that continues long after the initial infections have passed.

One of the obstacles preventing further study of HHV-6A in serum of MS patients is that current commercial assays are not sensitive enough to detect the virus in cases of low-grade chronic infection (See Testing). However, four studies in the late 1990s (when such an assay was briefly available), showed dramatic differences between patients with MS and controls without MS.

“Molecular mimicry” involving HHV-6 has been proposed as one mechanism by which the autoimmune process could be triggered and eventually progress toward the development of MS.  One study showed that certain residues on the HHV-6 genome are identical to residues of myelin basic protein.  Importantly, both T-cells and antibody responses to this peptide sequences were found elevated in MS patients (Tejada-Simon 2003). Moreover, in vitro infection of glial precursor cells was found to impair cell replication and increase the expression of oligodendrocyte markers, suggesting that HHV-6 infection of the CNS may influence the neural repair mechanism (Dietrich 2004).

The leading theory of CMV-induced autoimmunity in transplant patients is that cell surface proteins from CMV-infected tissues are incorporated into the viral envelope of CMV, inducing graft-versus-host disease post transplant. Similarly, an argument could be made that myelin proteins from infected oligodendrocytes could become incorporated into the HHV-6A envelope as they enter and leave the cell, inducing CNS autoimmunity in MS and CFS patients.

Recently, many studies have suggested HHV-6A as a factor in a subset of patients with both CFS and MS. Studies using assays that differentiate between active and latent virus have shown an exceptionally strong association between HHV-6A and both MS and CFS. We now know that due to the high level of latent virus found in controls, active infection should be measured by looking for HHV-6 DNA in cell free serum or plasma. This explains why there has been confusion about the association of HHV-6A with CFS in the past. The negative studies were done primarily with testing methods that did not differentiate latent from active virus.

In MS, 88% of patients complain of moderate to severe fatigue. As in CFS, MS patients also suffer from impaired information processing speed, enlarged cerebral ventricular volumes, and altered glucose metabolism in the prefrontal cortex. Okada et al (Okada 2004) have reported an 11% reduction in prefrontal gray matter by morphometric analysis in CFS patients; a pattern also found in MS. HHV-6A DNA has been demonstrated in the cerebral spinal fluids, plaques and other neural cells in both conditions. This has led some scientists to speculate that the two disorders share a common etiology.

Antiviral therapies are already used successfully for both MS (inferferon, amantadine) and CFS (ampligen). Once active HHV-6A infections can be diagnosed in CFS and MS patients through the use of more sensitive assays in commercial laboratories, these conditions may be treatable to some degree with antiviral and immune modulators. Such treatment has the potential to relieve fatigue and improve CNS function in patients with HHV-6A viremia.

Read Kristin Loomis’ recent post calling for a clinical trial due to ample evidence that HHV-6A triggers MS in a significant subset.

Key Papers: HHV-6 & Multiple Sclerosis

Cheng

2012

Cross-reactivity of autoreactive T cells with MBP and viral antigens in patients with MS
Nora-Krukle

2011

HHV-6 & HHV-7 reactivation and disease activity in multiple sclerosis
Khaki

2011

Evaluation of viral antibodies in Iranian multiple sclerosis patients.
Vandenbroeck

2011

Validation of IRF5 as multiple sclerosis risk gene: putative role in interferon beta therapy and human herpes virus-6 infection.
Garcia-Montojo

2011

Human herpesvirus 6 and effectiveness of interferon beta 1b in multiple sclerosis patients.
Behzad-Behbahani

2011

Human herpesvirus-6 viral load and antibody titer in serum samples of patients with multiple sclerosis.
Virtanen

2011

Intrathecal human herpesvirus 6 antibodies in multiple sclerosis and other demyelinating diseases presenting as oligoclonal bands in cerebrospinal fluid.
Behzad-Behbahani

2009

Human herpesvirus-6 viral load and antibody titer in serum samples of patients with multiple sclerosis.
Ahram

2009

Association between human herpesvirus 6 and occurrence of multiple sclerosis among Jordanian patients.
Alvarez-Lafuente

2008

Herpesviruses and human endogenous retroviral sequences in the cerebrospinal fluid of multiple sclerosis patients.
Martínez

2007

Environment-gene interaction in multiple sclerosis: human herpesvirus 6 and MHC2TA.
Alvarez-Lafuente

2006

Clinical parameters and HHV-6 active replication in relapsing-remitting multiple sclerosis patients.
Hollsberg

2005

Presence of Epstein-Barr virus and human herpesvirus 6B DNA in multiple sclerosis patients: associations with disease activity.
Derfuss

2005

Intrathecal antibody (IgG) production against human herpesvirus type 6 occurs in about 20% of multiple sclerosis patients and might be linked to a polyspecific B-cell response.
Alvarez-Lafuente

2004

Relapsing-remitting multiple sclerosis and human herpesvirus 6 active infection.
Dietrich

2004

Infection with an endemic human herpesvirus disrupts critical glial precursor cell properties.
Okada

2004

Mechanisms underlying fatigue: a voxel-based morphometric study of chronic fatigue syndrome.
Tejada-Simon

2003

Cross-reactivity with myelin basic protein and human herpesvirus-6 in multiple sclerosis.
Chapenko

2003

Correlation between HHV-6 reactivation and multiple sclerosis disease activity.
Goodman

2003

Human herpesvirus 6 genome and antigen in acute multiple sclerosis lesions.
Berti

2002

Increased detection of serum HHV-6 DNA sequences during multiple sclerosis (MS) exacerbations and correlation with parameters of MS disease progression.
Soldan

2000

Increased lymphoproliferative response to human herpesvirus type 6A variant in multiple sclerosis patients.
Ablashi

2000

Frequent HHV-6 reactivation in multiple sclerosis (MS) and chronic fatigue syndrome (CFS) patients.
Ablashi

1998

Human Herpesvirus-6 (HHV-6) infection in multiple sclerosis: a preliminary report.
Soldan

1997

Association of human herpes virus 6 (HHV-6) with multiple sclerosis: increased IgM response to HHV-6 early antigen and detection of serum HHV-6 DNA.
Challoner

1995

Plaque-associated expression of human herpesvirus 6 in multiple sclerosis.
Patnaik

1995

Prevalence of IgM antibodies to human herpesvirus 6 early antigen (p41/38) in patients with chronic fatigue syndrome.


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